identifies in vivo protein-protein interactions of a bait protein of choice and proteins expressed from a cDNA library determines the protein binding regions of two proteins known to interact
positive colonies fluoresce bright green - therefore, one-step selection of potential positives under UV light
no time-consuming beta-galactosidase lift assays as essential in most conventional two-hybrid assays
screens for potentially toxic interacting proteins using galactose-inducible expression of libraries
